The film centers on the character of Rayne (BloodRayne), an unholy breed of human and vampire known as a dhampir. Dhampir are unaffected by crucifixes and do not thirst for human blood, but maintain a vampire weakness to holy water. She is the daughter of the Vampire King, Kagan, who has gathered an army of thralls, both vampire and human, in order to annihilate the human race. She was conceived when Kagan raped her mother, and she later witnessed him killing her when she rejected his advances.