The film is set a few days after the end of the TV Series. A new strain of the Bugster Virus spreads among the population and a group of Ninja Players led by Kamen Rider Fuma invades the Seito University Hospital. Emu, Poppy, Hiiro and Taiga transform into Kamen Riders to fight back, but are defeated, and everybody except for Emu is infected and put into a coma, with Fuma using his Gashacon Bugvisor on a child patient with brain tumor called Madoka Hoshi and rendering her also unconscious before leaving. Once she awakes, Poppy is surprised to see her friends participating in a School Festival, as if nothing had happened. Meanwhile, Kiriya and Kuroto start to investigate the origin of this new virus and discover through Genm Corp's new president Tsukuru Koboshi that Masamune once negotiated with Johnny Maxima, the CEO of the game company Machina Vision regarding the contents of Kamen Rider Chronicle, but the deal was never completed, and both a Rider Gashat and Gamer Driver was stolen a few days later, along part of the company's data.