A group of martial arts and combat masters are invited to a fighting contest, "Dead or Alive", on an isolated island within an advanced complex, with the ultimate prize of $10 million. Among the competitors are Kasumi, a Ninja ninja-princess looking for her brother Hayate (who was competing in the last tournament), Tina, a professional wrestler setting out to prove she has more potential (complicated by her father Bass being one of the contenders), Christie, a master thief and assassin, her treacherous partner Maximillian ‘Max’ Marsh, and Hayabusa, a friend of Kasumi and Hayate who follows Kasumi to keep her safe, using the invitation to DOA for this. A final competitor is Helena Douglas, daughter of the tournament’s late founder. When they arrive, they are monitored by the island’s supervisor, Dr. Victor Donovan, who, aided by egghead Weatherby, is gathering data (using injected nano-sensors) from the fights for some mysterious project. To add to the situation, an assassin from Kasumi's colony, named Ayane, has followed Kasumi to kill her and wipe away the disgrace the princess has caused to the clan.