After the supervillain Gru adopted the three orphans Margo, Edith and Agnes, a lot has changed in his life. Instead of committing crimes as a curmudgeon, his life now revolves around bedtime stories, children's birthday parties and the girls' first suitors. His new life is suddenly disrupted when Lucy, an agent of the secret Anti-Villain League, takes Gru to their headquarters against his will. There, Gru is given the task of saving the world from a new villain. Gru eagerly sets to work and once again gets help from Dr Nefario and his usually very clumsy Minions. But reconciling his new task with bringing up his adopted daughters is not easy, and the fact that Lucy is also very fond of Gru outside of work is too much for the former loner.